What I understand so far is that DNA is isolated and then added to a vector so that it can be added to host cells (bacteria). I understand the whole process and how this is done and how to identify which bacteria have taken the vector and the rest.
Now what I dont understand is how the gene is added from the bacteria (host cell) to the the plant once they have been cloned, can anyone explain please? Eg, say the gene for resistant to herbicides is isolated and added to vector then into bacteria, bacteria are identified and grown. Then what happens?
Also, can the host cell be anything or does it have to be bacteria?
- Study Helper
Secondly, the gene that is inserted in bacterial plasmid also increases in number as bacterial cells divide. Now conditions can be made ambient for the gene to express itself and make a protein product. Or the plasmid may be spliced up, and the gene identified by a probe and then isolated.
Edit: As Dynamo said, for the transformation of plants Agrobacterium is mainly used but for mammalian systems it is usually viral vectors.