carla1
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Right im a bit confused about 2 things!
Firstly, serial dilution.
In my text book, it says, if you've diluted the sample ten-fold, then the concentration is 0.1 (or 10 to the power of -1) and the dilution factor was 10.
Then in a question, the dilution factor was 0.1.
can anyone tell me if the dilution factor increases or decreases with successive dilutions?

Also,
In penicillin production, my text book states 'the microbes grow rapidly and enzymes do not have to be supplied. This means that fermentation can take place at lower temperatures than normally used in industrial processes'
Can anyone help me? I dont understand why not using enzymes= lower temps??
I thought USING enzymes allowed reactions to take place at lower temperatures?
Thank you!
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Eloades11
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(Original post by carla1)
Right im a bit confused about 2 things!
Firstly, serial dilution.
In my text book, it says, if you've diluted the sample ten-fold, then the concentration is 0.1 (or 10 to the power of -1) and the dilution factor was 10.
Then in a question, the dilution factor was 0.1.
can anyone tell me if the dilution factor increases or decreases with successive dilutions?

Also,
In penicillin production, my text book states 'the microbes grow rapidly and enzymes do not have to be supplied. This means that fermentation can take place at lower temperatures than normally used in industrial processes'
Can anyone help me? I dont understand why not using enzymes= lower temps??
I thought USING enzymes allowed reactions to take place at lower temperatures?
Thank you!
1) If you increase the dilution factor, you lower the concentration even further.

2) It seems you're a bit confused in your interpretation. The increased abundance of microbes (rapidly reproductive microbes) means an increase in fermentation enzymes. This means it can take place at lower temperatures, but the enzyme kinetics/reaction will happen a lot slower than at higher temperatures.
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