jasy15
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Briefly outline how enzyme activity is affected by changes in pH, temperature, and salt concentration
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Solivagant
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We're not here to do your homework for you.

Which bits are you struggling with?
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Eloades11
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(Original post by jasy15)
Briefly outline how enzyme activity is affected by changes in pH, temperature, and salt concentration
As CJG21 said, we're not here to do your homework for you. At least provide your thoughts on the question so we can help you with your understanding. This looks like it can be answered with a quick visit to Wikipedia.
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zed963
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High change in ph causes it to become denatured.


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mynameisntbobk
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(Original post by zed963)
High change in ph causes it to become denatured.


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I don't believe pH affects the shape of the active site. Its due to partial charges on the active site. At lower pHs, negative areas are 'blocked' by protons, which reduces the ability of the enzyme to induce a fit around the substrate, reducing enzyme activity.
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zed963
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(Original post by mynameisntbobk)
I don't believe pH affects the shape of the active site. Its due to partial charges on the active site. At lower pHs, negative areas are 'blocked' by protons, which reduces the ability of the enzyme to induce a fit around the substrate, reducing enzyme activity.
You may be right but as far as my biol 1 knowledge I've been taught that changing the concentration of hydrogen ions disrupts the ionic bonding in the tertiary structure. Causing it to unravel and become denatured.


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thegodofgod
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(Original post by mynameisntbobk)
I don't believe pH affects the shape of the active site. Its due to partial charges on the active site. At lower pHs, negative areas are 'blocked' by protons, which reduces the ability of the enzyme to induce a fit around the substrate, reducing enzyme activity.
I'd have thought that pH would affect the ionisation of the amino acids (with charged side chains) within the polypeptide, which would affect the ionic bonds. This could lead to a change in the tertiary structure of the polypeptide, which may or may not, therefore, affect the structure of the active site.
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mynameisntbobk
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(Original post by zed963)
You may be right but as far as my biol 1 knowledge I've been taught that changing the concentration of hydrogen ions disrupts the ionic bonding in the tertiary structure. Causing it to unravel and become denatured.


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(Original post by thegodofgod)
I'd have thought that pH would affect the ionisation of the amino acids (with charged side chains) within the polypeptide, which would affect the ionic bonds. This could lead to a change in the tertiary structure of the polypeptide, which may or may not, therefore, affect the structure of the active site.

Yeah, I'm probably wrong, just ignore me
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Eloades11
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(Original post by mynameisntbobk)
Yeah, I'm probably wrong, just ignore me
The method of bonding isn't really necessary for this question anyway all you need to know is that the extreme pH's usually cause denaturation (irreversible disruption) of enzyme activity. Here a graph representing the average activity levels for an average enzyme at different pH's.
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