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    Hi,

    This is part of the question:

    'To make the dilution of the filtrate, the volume of the filtrate (V1) must be calculated using the dilution factor of 1 in 500 given above.

    Calculate the volume of filtrate that you will need to add to the volumetric flask (25mL) for a 1 in 500 dilution. '

    The answer I got for this was 50 microlitres, which is correct as I had to make this up in class.

    I then had to measure the absorbance of a sample solution at 250nm which gave me an answer of 1.220

    I am now being asked for the concentration of dilution, how do I do this? The dilution factor is 1 in 500.
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    (Original post by Pacman1910)
    Hi,

    This is part of the question:

    'To make the dilution of the filtrate, the volume of the filtrate (V1) must be calculated using the dilution factor of 1 in 500 given above.

    Calculate the volume of filtrate that you will need to add to the volumetric flask (25mL) for a 1 in 500 dilution. '

    The answer I got for this was 50 microlitres, which is correct as I had to make this up in class.

    I then had to measure the absorbance of a sample solution at 250nm which gave me an answer of 1.220

    I am now being asked for the concentration of dilution, how do I do this? The dilution factor is 1 in 500.
    Don't you have to measure the concentration on the x-axis and the absorbance on the y-axis for a few different concentrations, then create a line or curve of best fit, and then either extrapolate if the absorbance isn't within the range of concentrations that you've selected?
 
 
 
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