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1H 15N HSQC Protein NMR

Can someone explain this in a more accessible way please :smile:
Original post by flyylikejetz
Can someone explain this in a more accessible way please :smile:


Have you read through this
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goose183
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Original post by flyylikejetz
Can someone explain this in a more accessible way please :smile:



I'll try to expain WHY it is done rather that how, as the wiki page expains that. Basically, (very basically) it is the results of a proton NMR and 15N NMR plotted against eachother on a graph. The extra information you get from this is that the protons and nitrogens show up as bonded pairs. Whereas if you just did 2 separate NMR experiments, it would be difficult to show which H corresponds to which N. So each peak on the HSQC shows a N-H bonded pair, rather than an individual nucleus. With molecules as complex as proteins, this saves a lot of time and frustration, because different amino acid residues have characteristic peaks on a 2-D NMR (in general).

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