Hey there! Sign in to join this conversationNew here? Join for free
    • Thread Starter
    Offline

    3
    ReputationRep:
    Hi guys, I was wondering if anyone could help me out with my bio coursework? Originally my experiment was going to be adding hydrogen peroxide to pieces of liver from different animals (e.g. pig, sheep, cow) as a way of measuring catalase activity.

    However, now I've come up with a slightly different experiment which I think would be better. I would add different ethanol concentrations to pieces of the same liver, and then add hydrogen peroxide to each. I think doing this experiment would be better because it removes all the confounding variables e.g. the diet, age etc. of the animals from which the livers came from.

    Would this work or would the alcohol have had to be ingested whilst the animals was still alive?

    Please let me know
    • Thread Starter
    Offline

    3
    ReputationRep:
    Anyone?
    • PS Reviewer
    • Study Helper
    Offline

    14
    ReputationRep:
    Moved to the Bio forum for you . You should get more responses here :yep:
    • Thread Starter
    Offline

    3
    ReputationRep:
    (Original post by Nirgilis)
    Moved to the Bio forum for you . You should get more responses here :yep:
    Thanks
    Offline

    13
    ReputationRep:
    (Original post by lilixxx1000)
    Hi guys, I was wondering if anyone could help me out with my bio coursework? Originally my experiment was going to be adding hydrogen peroxide to pieces of liver from different animals (e.g. pig, sheep, cow) as a way of measuring catalase activity.

    However, now I've come up with a slightly different experiment which I think would be better. I would add different ethanol concentrations to pieces of the same liver, and then add hydrogen peroxide to each. I think doing this experiment would be better because it removes all the confounding variables e.g. the diet, age etc. of the animals from which the livers came from.

    Would this work or would the alcohol have had to be ingested whilst the animals was still alive?

    Please let me know
    It is difficult to comment on this because you've not quite fully explained the investigation.

    Are you immersing the liver in hyrogen peroxide or dropping it on with a pippette? Adding too much/too little could distort your results so make sure you use equal volumes of HP on all samples of liver. Same regarding liver size, make sure all samples are the same size and that the size is appropriate. If tiny pieces of liver you may struggle to get good results without highly precise equipment, if huge great chunks then there may be other complications. I don't know for sure but just make sure the sizes are all the same in the name of consistency.

    How are you planning to measure the oxygen and water output of the catalase?

    Have you discussed this with your teacher as I would have thought that the kind of liver you will have access to may have had it's enzyme load already destroyed. Liver from supermarkets etc is often frozen or stored in unfavourable conditions, destroying enzymes. You should do what you can to make sure your sample liver is sourced as fresh as possible (from a local butcher maybe?) and is acquired as close to the start of the practical as possible, i.e the morning of the day the practical is on.

    Why are you considering adding ethanol? In order to reduce number and efficiency of catalase within the sample? To then observe the different catalase output relative to the amount of ethanol/catalase activity?

    I know this topic is old but I hope you see this in time! Good luck!
 
 
 
  • See more of what you like on The Student Room

    You can personalise what you see on TSR. Tell us a little about yourself to get started.

  • Poll
    What newspaper do you read/prefer?
  • See more of what you like on The Student Room

    You can personalise what you see on TSR. Tell us a little about yourself to get started.

  • The Student Room, Get Revising and Marked by Teachers are trading names of The Student Room Group Ltd.

    Register Number: 04666380 (England and Wales), VAT No. 806 8067 22 Registered Office: International House, Queens Road, Brighton, BN1 3XE

    Quick reply
    Reputation gems: You get these gems as you gain rep from other members for making good contributions and giving helpful advice.