I am super stuck on how to calculate standards within a linear range. I have no idea where to start. How would I calculate a protein standard solution of 1.0mg/ml with a linear range of 0.2-10mg/ml? How much of buffer/water/protein would I add?
Help! Really stuck
Protein standard calculation watch
- Thread Starter
- 18-01-2015 18:33
- 22-01-2015 14:39
Not 100% what you mean, but the buffer IS water itself + other things, so it's just a protein:buffer calculation.
You can add 10 mg protein to 1 ml, hence making a 10 mg/ml solution. Then dilute it by taking say 0.5 ml of it and making back up to 1 ml; this is now your 5 mg/ml solution. You can make any concentration from this 10 mg/ml stock, and adjust the volumes as necessary e.g. if you need 10 ml of 10mg/ml solution, just add 100 mg in 10 ml.
Does this help?