Whats the disadvantage in using HCL when precipitating plasma proteins? Watch

Tj789
Badges: 10
Rep:
?
#1
Report Thread starter 4 years ago
#1
It's a biology question, but I thought as chemistry links into it some of you may be able to help.

My answer:
Using HCL is could be a disadvantage because they have a low pH which can cause the active site of the enzymes change shape, this means that the enzyme will denature and use most of it if not all of its function. This is a huge disadvantage because the purpose of precipitating proteins is so that we can use them for medical purposes, but if they are denatured then we cannot use them.

Would appreciate it, tanx!
0
reply
Nirgilis
  • Study Helper
Badges: 14
Rep:
?
#2
Report 4 years ago
#2
(Original post by Tj789)
It's a biology question, but I thought as chemistry links into it some of you may be able to help.

My answer:
Using HCL is could be a disadvantage because they have a low pH which can cause the active site of the enzymes change shape, this means that the enzyme will denature and use most of it if not all of its function. This is a huge disadvantage because the purpose of precipitating proteins is so that we can use them for medical purposes, but if they are denatured then we cannot use them.

Would appreciate it, tanx!
I think you've pretty much got it :yep: . The key here is the pH. If it drops out of biological range, then the protein will denature. As you've said, this means it will lose it's shape and structure and hence lose function.

However, precipitated proteins are used for a lot more than medical purposes, but I don't know how much detail you need to go into
1
reply
X

Quick Reply

Attached files
Write a reply...
Reply
new posts
Latest
My Feed

See more of what you like on
The Student Room

You can personalise what you see on TSR. Tell us a little about yourself to get started.

Personalise

University open days

  • University of Birmingham
    Postgraduate Open Day Postgraduate
    Wed, 20 Mar '19
  • King's College London
    Postgraduate Taught Courses - Arts & Sciences - Strand Campus Postgraduate
    Wed, 20 Mar '19
  • University of East Anglia
    All Departments Open 13:00-17:00. Find out more about our diverse range of subject areas and career progression in the Arts & Humanities, Social Sciences, Medicine & Health Sciences, and the Sciences. Postgraduate
    Wed, 20 Mar '19

Where do you need more help?

Which Uni should I go to? (18)
12.16%
How successful will I become if I take my planned subjects? (10)
6.76%
How happy will I be if I take this career? (35)
23.65%
How do I achieve my dream Uni placement? (22)
14.86%
What should I study to achieve my dream career? (16)
10.81%
How can I be the best version of myself? (47)
31.76%

Watched Threads

View All