Making hyper, iso and hypotonic solutions to add to blood?

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Michael1997
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Report Thread starter 7 years ago
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In my biology investigation my first aim is to investigate the effect of adding hyper, iso and hypotonic solutions to blood; I have acquired pig blood from my local butchers, although I can't seem to find anywhere that has instructions on how to synthesis said solutions.

Can someone please include instructions of chemicals I require, and steps!
Thank you.
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loukas2993
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Report 6 years ago
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(Original post by Michael1997)
In my biology investigation my first aim is to investigate the effect of adding hyper, iso and hypotonic solutions to blood; I have acquired pig blood from my local butchers, although I can't seem to find anywhere that has instructions on how to synthesis said solutions.

Can someone please include instructions of chemicals I require, and steps!
Thank you.
This seems like an odd independent investigation to run; I would have thought you would have been provided with 'blood' and any hyper, hypo or isotonic solutions you may need. But here goes:

Hypertonic solution - can be any plain glucose solution with a concentration that exceeds 5%. For example, 10% dextrose in water, also known by its name D10W (i.e. this is often used as an IV fluid)
Hypotonic solution - can be any plain glucose solution with a concentration lower than 5%.
Isotonic solution - can be any plain glucose solution with a 5% concentration.

Essentially, I am basing these concentrations on plasma osmolality.

Hope this helps.
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