Uses of DNA in science and Technology essay

Watch this thread
debonington
Badges: 0
Rep:
? You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#1
Report Thread starter 7 years ago
#1
Has anybody done this essay? I'm really struggling on it
0
reply
elizatuesday
Badges: 1
Rep:
? You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#2
Report 7 years ago
#2
doing it now
my plan is this:

• Screen dna
o Probes
 Locate mutated genes
 Sequence gene
 Produce multiple probes (PCR)
 Label the probe
 Incubate with dnd ad electrophoresis
 OR
 Microarray
 Labelled DNA washed over many of the same probe
 Will only stick if has mutated gene
 Many different genes screened at same time

• Gene therapy (treatment)
o somatic
 Cycstic fibrosos
 CFTR gene inserted into lungs
o Germ line therapy
 Screening of IVF embroys

• Cancer treatment
o Breast c mutation on HER2 proto-oncogene treated by Herceptin
o Binds to HER2 receptor to stop growth
o Skin c mutation of B-RAF protoonco
o treated by ZELBORAF
o Inhibits braf enzyme which stops cells that express it from growing
o Breast, pancreatic, cervical caused by mutation on BRCA
o small molecules blocks enzymes that repair DNA and kills cell


• other uses of PCR (in vitro)
o Crime scenes
o Genetic fingerprinting/electrophoresis
 Genetic relationships
• Between species classification
• Separation temp experiment
 Diagnosis – genetic disorders

• In vivo cloning
o Recombinant and transformed cells
o Plasmids or viruses
o Genetic engeneering
 Makes proteins – insulin for diabetes
 Bacterium used as vector to insert transformed into plant e.g. tolerate drought
• Golden rice
o Beta carotene helps vitamin A deficiency
• Biological catalysts
o Chymosin enzyme for cheese production
• Vaccines
o Most need refrigeration
o Some countries cant
- allows for production of vaccines that dont need refrigeration


• Stem cells human
o Make genetically identical organs/tissues bodies wont reject


• Stem cells plant
o Totipotent
o Organ or clone

hope this helps - ask if you want clarification or explanation of examples
0
reply
rouzbehrh
Badges: 4
Rep:
? You'll earn badges for being active around the site. Rep gems come when your posts are rated by other community members.
#3
Report 7 years ago
#3
I do a course on this in uni so to nerd it j would recommend writing about omics, transciptomics like microarray a to detect expression levels, rna seq also, Sanger sequencing followed by deep sequencing, massively parallel etc


Posted from TSR Mobile
1
reply
X

Quick Reply

Attached files
Write a reply...
Reply
new posts
Back
to top
Latest
My Feed

See more of what you like on
The Student Room

You can personalise what you see on TSR. Tell us a little about yourself to get started.

Personalise

Year 12s - where are you at with making decisions about university?

I’ve chosen my course and my university (22)
28.95%
I’ve chosen my course and shortlisted some universities (27)
35.53%
I’ve chosen my course, but not any universities (7)
9.21%
I’ve chosen my university, but not my course (3)
3.95%
I’ve shortlisted some universities, but not my course (4)
5.26%
I’m starting to consider my university options (10)
13.16%
I haven’t started thinking about university yet (2)
2.63%
I’m not planning on going to university (1)
1.32%

Watched Threads

View All
Latest
My Feed