F215 How to sequence a genomeWatch
My CGP revision guide says that you have to use BAC's (bacterial artificial chromosomes), but this seems unnecessarily complicated and I don't see the point of it! Why can't you just use PCR? If you're sequencing a genome, why is this any different to just sequencing lots of DNA? My textbook doesn't mention BAC's at all, and my teacher told me that she'd never heard of them, and to just ignore that section in the book. I also can't find any past paper questions on it, so I don't even know whether I'm supposed to be learning about BAC's!
So if someone could tell me how OCR would like me to answer a question about how to sequence a genome, that would be great!
I'm sorry if there are other threads about this, but I couldn't find any! Any help would be really appreciated
I don't do OCR so I won't be that much help... but PCR is just a method of amplifying DNA fragments, so while it can be useful in sequencing to create many copies of a fragment, it won't actually be used to determine the base sequence by itself.