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AS Biology Coursework Help - Enzyme Neutrase and Casein

Does anyone know where I can find a 'model plan' to base my biology coursework around. My investigation is to measure the absorbancy of light of different concentrations of casein solution mixed with the enzyme neutrase within a specific time frame/temp and thus deduce whether altering substrate concentration has an effect on enzyme activity.

Casein solution is white in colour and when the enzyme neutrase is added the proteins are hydrolyzed and eventually the solution turns colourless.

I haven't studied Biology in years and am not sure how to go about making a plan.

Many Thanks!

p.s. a calibration curve needs to be made. Is this done by testing the light absorbancy of various concentrations of substrate + a known volume of water (i.e a substitute for same volume of clear enzyme)? Does a calibration curve give an idea of the 'starting point' light absorbancy of the solutions?

Sorry, I had my first class last week and things aren't 100% clear to me

Reply 1

chickpea

This sounds very similar to the coursework I am doing - ' the effect of temperature on the enzyme Trypsin when hydrolising Casein'

The first thing needed though is to ask what board and spec you are doing this coursework for and can you change the investigation as concentrations are quite difficult to get good data, whereas temperature is a bit easier. :smile: