SallyLouise
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#1
Report Thread starter 6 years ago
#1
Can someone please help me with this question:

Research and write-up methods for testing for biological molecules: Starch, protein, lipids, reducing sugar and non-reducing sugar.
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Treblebee
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#2
Report 6 years ago
#2
The Internet is your best friend

(Btw, didn't they mention iodine for starch, the piece-of-paper test for lipids, etc?)
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chocolatecookieM
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#3
Report 6 years ago
#3
The test for proteins, is add an equal volume of biuret reagent to a liquid food sample (2cm3) and then if it stays blue the result is negative if it turns purple the result is positive.
Whens the homework due in?
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Rorschach II
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#4
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#4
If you @ me, I might be able to find the time to scan in my notes tomorrow or something.
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KingAuthor
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#5
Report 6 years ago
#5
Make sure you know these tests off by heart

Reducing sugar - Add Benedict's reagent to sample / heat / brick red precipitate if reducing sugar present.

Non reducing sugar - Add HCl to fresh sample / Heat / neutralise / test with Benedict's reagent / brick red precipitate as non reducing has been hydrolysed into reducing

Starch - iodine test / blue Black if starch present

Protein - biuret reagent / violet if proteins are present (this test will also test positive for ENZYMES)

Lipids - Add solution and shake / add water / mily/cloudy white emulsion formed if lipids are present.

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