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# Concentration/ Dilution Question? watch

1. Hi, having trouble with this if anyone can help this is an assay to work out the concentration of atenolol in tablets:

"Powder 20 tablets. Transfer the powder to a 500 mL flask using 300 mL of methanol, heat the resulting suspension to 60° and shake for 15 minutes. Cool, dilute to 500 mL with methanol, filter through a fine glass micro-fibre filter paper (Whatman GF/C is suitable) and dilute a suitable volume of the filtrate with sufficientmethanol to produce a solution containing 0.01% w/v of Atenolol. Measure the absorbance of the resulting solution at the maximum at 275 nm, Appendix II B. Calculate the content of C14H22N2O3 taking 53.7 as the value of A(1%, 1 cm) at the maximum at 275 nm"

How do I work out what the "suitable volume" is to produce a solution containing 0/01% w/v?Also what will the final volume be?

Thank you for any help and/ explanation!
Hi, having trouble with this if anyone can help this is an assay to work out the concentration of atenolol in tablets:

"Powder 20 tablets. Transfer the powder to a 500 mL flask using 300 mL of methanol, heat the resulting suspension to 60° and shake for 15 minutes. Cool, dilute to 500 mL with methanol, filter through a fine glass micro-fibre filter paper (Whatman GF/C is suitable) and dilute a suitable volume of the filtrate with sufficientmethanol to produce a solution containing 0.01% w/v of Atenolol. Measure the absorbance of the resulting solution at the maximum at 275 nm, Appendix II B. Calculate the content of C14H22N2O3 taking 53.7 as the value of A(1%, 1 cm) at the maximum at 275 nm"

How do I work out what the "suitable volume" is to produce a solution containing 0/01% w/v?Also what will the final volume be?

Thank you for any help and/ explanation!
The answer is inaccessible without the mass of atenolol in a tablet ...
3. (Original post by charco)
The answer is inaccessible without the mass of atenolol in a tablet ...
Ahh sorry I forgot to add that...25mg!
Ahh sorry I forgot to add that...25mg!
20 tabs = 500mg

The solution you make in the instructions has a volume of 500 ml

Hence 1 mg/ml = 1g/L

this corresponds to a 0.1 % solution (1/1000)

To make a 0.01 % solution you need to do a 1:10 dilution.

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