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    Hi, having trouble with this if anyone can help this is an assay to work out the concentration of atenolol in tablets:

    "Powder 20 tablets. Transfer the powder to a 500 mL flask using 300 mL of methanol, heat the resulting suspension to 60° and shake for 15 minutes. Cool, dilute to 500 mL with methanol, filter through a fine glass micro-fibre filter paper (Whatman GF/C is suitable) and dilute a suitable volume of the filtrate with sufficientmethanol to produce a solution containing 0.01% w/v of Atenolol. Measure the absorbance of the resulting solution at the maximum at 275 nm, Appendix II B. Calculate the content of C14H22N2O3 taking 53.7 as the value of A(1%, 1 cm) at the maximum at 275 nm"

    How do I work out what the "suitable volume" is to produce a solution containing 0/01% w/v?Also what will the final volume be?

    Thank you for any help and/ explanation!
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    (Original post by AboutThatLife93)
    Hi, having trouble with this if anyone can help this is an assay to work out the concentration of atenolol in tablets:

    "Powder 20 tablets. Transfer the powder to a 500 mL flask using 300 mL of methanol, heat the resulting suspension to 60° and shake for 15 minutes. Cool, dilute to 500 mL with methanol, filter through a fine glass micro-fibre filter paper (Whatman GF/C is suitable) and dilute a suitable volume of the filtrate with sufficientmethanol to produce a solution containing 0.01% w/v of Atenolol. Measure the absorbance of the resulting solution at the maximum at 275 nm, Appendix II B. Calculate the content of C14H22N2O3 taking 53.7 as the value of A(1%, 1 cm) at the maximum at 275 nm"

    How do I work out what the "suitable volume" is to produce a solution containing 0/01% w/v?Also what will the final volume be?

    Thank you for any help and/ explanation!
    The answer is inaccessible without the mass of atenolol in a tablet ...
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    (Original post by charco)
    The answer is inaccessible without the mass of atenolol in a tablet ...
    Ahh sorry I forgot to add that...25mg!
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    (Original post by AboutThatLife93)
    Ahh sorry I forgot to add that...25mg!
    20 tabs = 500mg

    The solution you make in the instructions has a volume of 500 ml

    Hence 1 mg/ml = 1g/L

    this corresponds to a 0.1 % solution (1/1000)

    To make a 0.01 % solution you need to do a 1:10 dilution.
 
 
 
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