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Yeast Respiration Question Watch

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    I'm doing my Biology IA on yeast respiration rate in different sugars and I'm doing a t-test to find out whether my hypothesis is valid or invalid. However, I am unsure whether I should use an independent variable t-test or a dependent variable t-test.
    Considering the subject being tested is yeast and yeast is almost always exactly the same save for size or weight variations of the active dry yeast granules, and the variable that is being tested is the rate of respiration ( in ppm/s ) in different sugars, does this make these two samples dependent or independent.
    In a way they are independent as the two samples don't tell anything about each other, so if the respiration rate under glucose is so and so, that does not mean anything about the respiration rate under say sucrose.
    However, in another way, they are dependent as the yeast is always yeast and doesn't really vary, thus it is simply yeast being tested under different conditions, those being different types of sugars.
    I'm really unsure on which t-test to use for this experiment...
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