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    I understand Beer-Lambert Law is A=εlc, but am not sure how to use it.

    I have recorded at a set of absorbancies in OD/min and would like to convert them into μM/minute.

    e.g. one of my absorbancies is 0.35, how would I covert this into μM/minute, what other values do I need to use?
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    (Original post by AryaStark)
    I understand Beer-Lambert Law is A=εlc, but am not sure how to use it.

    I have recorded at a set of absorbancies in OD/min and would like to convert them into μM/minute.

    e.g. one of my absorbancies is 0.35, how would I covert this into μM/minute, what other values do I need to use?
    You need the molar extinction coefficient of the solution that you are analysing.
    It is easier to use a calibration curve.
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    (Original post by charco)
    You need the molar extinction coefficient of the solution that you are analysing.
    It is easier to use a calibration curve.
    AHh, thank you!!!
 
 
 
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