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    Hi all

    Im new here. I am studying Biological Sciences at Uni after doing an access course at college. I am definitely a mature student and a bit of a late starter with Science. So some things I become easily stuck on.

    So if anyone can help me I would be appreciated. The info below is the practical we have just done. It then asks to do a graph finding the 50% lysis of cells. This is where I am stuck. I have the percentage of each tube and the guinea pig serum on x and y axis. What I need to know is do I do it this way or do I use absorbance and include my 100% lysed tube and my true OD tube aswell. Or is my graph complete just including 3 tubes WITH serum and their percentage????
    I am soooo dizzy and often wonder how I remember my name, so will appreciate any help, even is its to direct me to the right reading material as I am also struggling to find info.
    Complement proteins are present in the blood sera. They act in combination with antibodies to provide an efficient killing mechanism for the humoral mediated arm of the acquired immune system. Antibodies bind to the invading pathogen and this triggers initiation of the complement response.

    Due to the safety aspects of handling pathogens in undergraduate practicals, we are going to use sheep red blood cells (erythrocytes) as an example of foreign cells which have invaded an organism.
    You will be given some sheep erythrocytes which have been sensitised by adding an antibody which has attached to them (rabbit anti-sheep red blood cell antibody). You are then going to carry out complement-mediated lysis of these erythrocytes by adding various amounts of guinea pig serum, which contains all the complement proteins. You are going to ascertain the degree of lysis of the erythrocytes by recording the absorbance of each sample at 541nm.

    Thanks Sam
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Updated: January 24, 2016
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