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    Hi!

    I recently did a lab involving the Enzymic hydrolysis of Glycogen with amylase, cellulase and HCL.

    One of the questions I need to answer is; What use should be put of the controls values before plotting a graph?

    I'm unsure whether this question just doesn't make sense or if I'm not thinking it through?

    Any help is greatly appreciated.

    Thank you.
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    (Original post by JustBennify)
    Hi!

    I recently did a lab involving the Enzymic hydrolysis of Glycogen with amylase, cellulase and HCL.

    One of the questions I need to answer is; What use should be put of the controls values before plotting a graph?

    I'm unsure whether this question just doesn't make sense or if I'm not thinking it through?

    Any help is greatly appreciated.

    Thank you.
    Yeah the question is a bit badly structured grammatically. But what I think they're getting at is that you would need to use the data from the control experiments as a comparison. This is because glycogen would be subject to some degree of spontaneous (non-enzymatic hydrolysis) so the basal value isn't zero.
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    (Original post by Asklepios)
    Yeah the question is a bit badly structured grammatically. But what I think they're getting at is that you would need to use the data from the control experiments as a comparison. This is because glycogen would be subject to some degree of spontaneous (non-enzymatic hydrolysis) so the basal value isn't zero.
    Thank-you for your reply

    Wouldn't that fall under this question though? Explain the low values obtained for some of thecontrols (i.e. why are they not all zero?).

    Or would that be due to every solution having a certain absorbency level?

    EDIT: I think I'm just confusing myself. I'm sorry.
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    (Original post by JustBennify)
    Thank-you for your reply

    Wouldn't that fall under this question though? Explain the low values obtained for some of thecontrols (i.e. why are they not all zero?).

    Or would that be due to every solution having a certain absorbency level?

    EDIT: I think I'm just confusing myself. I'm sorry.
    Yes that's the reason. Everything will have a low level absorbance but the spectrophotometer should be calibrated around a blank reading.

    What I think it is, is that you would measure the ratio of actual to control instead of just the raw value for the actual
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    (Original post by Asklepios)
    Yes that's the reason. Everything will have a low level absorbance but the spectrophotometer should be calibrated around a blank reading.

    What I think it is, is that you would measure the ratio of actual to control instead of just the raw value for the actual
    So just to confirm,

    Explain the low values obtained for some of thecontrols (i.e. why are they not all zero?). - The reasoning behind the absorbance values for the controls is due to every solution having a certain level of absorbance.

    What use should be put of the controls values before plotting a graph? - You would need to usethe data from the control experiments as a comparison; this is because glycogenwould be subject to some degree of spontaneous (non-enzymatic hydrolysis) sothe basal value isn't zero. (rephrased into my own words of course)

    Thank you for your help once again, you've helped alot!
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    (Original post by JustBennify)
    So just to confirm,

    Explain the low values obtained for some of thecontrols (i.e. why are they not all zero?). - The reasoning behind the absorbance values for the controls is due to every solution having a certain level of absorbance.

    What use should be put of the controls values before plotting a graph? - You would need to usethe data from the control experiments as a comparison; this is because glycogenwould be subject to some degree of spontaneous (non-enzymatic hydrolysis) sothe basal value isn't zero. (rephrased into my own words of course)

    Thank you for your help once again, you've helped alot!
    The first one is because of spontaneous hydrolysis
 
 
 
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