Reaction mixture is set up which include DNA sample, free nucleotides, primers and DNA polymerase. DNA strand is separated by heating the mixture to 95 degrees to break hydrogen bonds between bases in DNA fragment. 2 separate strands are created. Addition of primer where the mixture is cooled to 55 degrees. The primers anneal to the complimentary bases at the end of each DNA fragment which provides the starting sequence for DNA polymerase to start synthesizing New DNA, The temperature is increased to 72 degrees which is optimum for DNA polymerase. The DNA polymerase adds complementary nucleotides to each of the two separated DNA strands. It begins at the primer on each strand then adds nucleotides until reaching the end of the chain
Can someone check my steps for PCR?? Biology x Watch
- Thread Starter
- 06-04-2016 11:36
- 06-04-2016 11:44
Yep, looks like you've got it!
I would just mention that when the temperature is increased to 72 degrees, it's optimum for TAQ DNA polymerase to make it slightly more specific. Otherwise, it's great!