Is Benedicts reagent a protein
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Last edited by Hollyd13; 17-04-2016 at 17:07.
- 17-04-2016 11:56
(Original post by Hollyd13)
- 17-04-2016 12:04
Is Benedicts reagent a protein? Also what would happen to the experiment of testing for reducing sugars if water from the water bath accidentally mixes with the Benedicts solution and the reducing sugars?
If it's a small amount of water, then probably nothing, however I'm not sure for larger volumes of water (the water could act as a ligand and replace NH3 ligand), but again I'm uncertain.
- 17-04-2016 12:07
Benedict's reagent is a solution of sodium citrate, sodium carbonate, and cupric sulphate pentahydrate, according to Wikipedia; when it's mixed with a solution containing reducing sugars, the cupric ion is reduced and precipitates as cuprous oxide, which is visibly red. So no, it's a mixture of ionic compounds, not a protein.
If water from the water bath gets into the solution, I suppose it would dilute the solution and might make it more difficult to discern the colour change? It wouldn't be that big of a problem, but you should still avoid it.Last edited by anosmianAcrimony; 17-04-2016 at 12:09.