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    Hello there

    I wonder if anyone may please be able to help me?

    If I have made a dilution of a substance by adding 300ul of solute to 300ul solvent and then taking 300ul of this and adding it to a further 300ul of solvent - doing this until I get a dilution of 1:16 (therefore repeating the dilution 4 times), and I want to go from the concentration of the solute at 1:16 back to the original concentration of the solute when it was neat, what should I do? For example, if I find the concentration of the solute at 1:16 to be 0.40mg/ml, how would I know what the concentration was when it was neat (before dilutions) should I not have a sample of the neat version anymore? Do I need to multiply the concentration at 1:16, e.g. the 0.40mg/ml, by something?

    Thank you very much.
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    If the final one was 16x more dilute than the stock solution, then the stock solution must have been 16x more conc. than the diluted one.

    Am I misunderstanding you?
 
 
 
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