hiv contains the enzyme reverse transcriptase genetic engineers have used this enzyme and mrna to produce a gene for human insulin
Describe how this gene could be produced
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- Thread Starter
- 14-05-2016 11:34
- 14-05-2016 11:39
Transcription takes DNA and makes it RNA. Reverse transcriptase therefore does the opposite: RNA to DNA. The thing about DNA fragments is that they can be inserted into the host DNA. Meaning if you put reverse transcriptase and purified insulin mRNA in a host and perhaps use an endonuclease to create a lesion at a place in the host's genome under a strong promoter, that means that the DNA made by reverse transcriptase is incorporated there. Which means your host that can be a baterium now has the gene for human insulin in its genome and it is producing the protein in large amounts.