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    Can anyone explain to me how the Benedict's test for non-reducing sugar works please? The procedure.
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    For the non-reducing sugar, you add 2cm3 of dilute hydrochloric acid (to 2cm3 of your sample); after having done this experiment once to confirm that there is no reducing sugar present.This hydrolyses the glycosidic bond; freeing the aldehyde and ketone groups from the non-reducing sugar.You will then heat in the water bath.Neutralise it with sodium hydrogen carbonate (test with litmus paper).Add 2cm3 of Benedict's solution to the heated sample; if it forms a coloured precipitate, a non-reducing sugar is present.
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    (Original post by Bigbosshead)
    For the non-reducing sugar, you add 2cm3 of dilute hydrochloric acid (to 2cm3 of your sample); after having done this experiment once to confirm that there is no reducing sugar present.This hydrolyses the glycosidic bond; freeing the aldehyde and ketone groups from the non-reducing sugar.You will then heat in the water bath.Neutralise it with sodium hydrogen carbonate (test with litmus paper).Add 2cm3 of Benedict's solution to the heated sample; if it forms a coloured precipitate, a non-reducing sugar is present.
    Thanks


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