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    Describe the formation of recombinant DNA?
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    (Original post by shawn gwerevende)
    Describe the formation of recombinant DNA?
    Depending what board you are on, I think it goes like this:

    obtain the desired gene by restriction endonuclease/reverse transcriptase
    amplify with in vivo cloning or PCR
    get vector: bacterial plasmid
    cut with same restriction endonuclease used to cut out gene
    sticky ends complementary and so bind
    DNA ligase to form phosphodiester bonds by condensation reactions to produce a plasmid with this new gene and thus this is recombinant DNA

    BTW I'm on AQA
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    (Original post by snmk007)
    Depending what board you are on, I think it goes like this:

    obtain the desired gene by restriction endonuclease/reverse transcriptase
    amplify with in vivo cloning or PCR
    get vector: bacterial plasmid
    cut with same restriction endonuclease used to cut out gene
    sticky ends complementary and so bind
    DNA ligase to form phosphodiester bonds by condensation reactions to produce a plasmid with this new gene and thus this is recombinant DNA

    BTW I'm on AQA
    Fine explanation. You got seven reputation points for that. Can't see something which you have forgotten to mention. Great!
 
 
 
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