shawn gwerevende
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Describe the formation of recombinant DNA?
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TSR Jessica
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Sorry you've not had any responses about this. Are you sure you've posted in the right place? Here's a link to our subject forum which should help get you more responses if you post there.

You can also find the Exam Thread list for A-levels here and GCSE here. :dumbells:


Just quoting in Puddles the Monkey so she can move the thread if needed
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username2434011
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(Original post by shawn gwerevende)
Describe the formation of recombinant DNA?
Depending what board you are on, I think it goes like this:

obtain the desired gene by restriction endonuclease/reverse transcriptase
amplify with in vivo cloning or PCR
get vector: bacterial plasmid
cut with same restriction endonuclease used to cut out gene
sticky ends complementary and so bind
DNA ligase to form phosphodiester bonds by condensation reactions to produce a plasmid with this new gene and thus this is recombinant DNA

BTW I'm on AQA
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Kallisto
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(Original post by snmk007)
Depending what board you are on, I think it goes like this:

obtain the desired gene by restriction endonuclease/reverse transcriptase
amplify with in vivo cloning or PCR
get vector: bacterial plasmid
cut with same restriction endonuclease used to cut out gene
sticky ends complementary and so bind
DNA ligase to form phosphodiester bonds by condensation reactions to produce a plasmid with this new gene and thus this is recombinant DNA

BTW I'm on AQA
Fine explanation. You got seven reputation points for that. Can't see something which you have forgotten to mention. Great!
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