# HELP Watch

1. if someone can give me an hint to how to proceed with this exercise I will appreciate that...thanks
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2. 00.docx (15.1 KB, 51 views)
3. (Original post by carmenbn)
if someone can give me an hint to how to proceed with this exercise I will appreciate that...thanks
Start off by plotting the graph of concentration vs velocity (you can calculate them using the Beer-Lambert law). You should find it to be a curve, and the velocity which the curve approaches can be estimated to be Vmax (i.e. the asymptote). Vmax/2 is Km.

Then plot the lineweaver-burk plot (1/V vs 1/substrate) and see if you can work out how to calculate Vmax and Km (think of the gradient and intercepts).
4. Thanks...but the major problem is that i'm confused about the units...in the exercise it is asked to change them !

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