HPLC, need help reading chromatograms

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    I carried out an experiment which resulted in a chromatogram with three peaks, now each peak belongs to one of three drugs. How can I associate each peak with its corrensponding drug?

    I have retention times of the drugs but cannot find any references online, if it helps they are aspirin, caffeine and paracetamol.
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    (Original post by SunTiger)
    I carried out an experiment which resulted in a chromatogram with three peaks, now each peak belongs to one of three drugs. How can I associate each peak with its corrensponding drug?

    I have retention times of the drugs but cannot find any references online, if it helps they are aspirin, caffeine and paracetamol.
    Use their respective polarity( degree of bonding to the stationary phase). The 'stickiest' will be the slowest, i.e. last to appear
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    (Original post by charco)
    Use their respective polarity( degree of bonding to the stationary phase). The 'stickiest' will be the slowest, i.e. last to appear
    And how would I do that? This is all I have got...

    http://imgur.com/a/9eMLY
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    (Original post by SunTiger)
    And how would I do that? This is all I have got...

    http://imgur.com/a/9eMLY
    You must know what the stationary phase is if you run a chromatography column ...

    http://lab-training.com/landing/free...g-programme-6/
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    (Original post by charco)
    You must know what the stationary phase is if you run a chromatography column ...

    http://lab-training.com/landing/free...g-programme-6/
    I know the phases, stationary, mobile etc... Also the retention time can show which drug is eluted faster, but how can I match the drug to the retention time? Am I missing something? Googleing didn't help much.
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    (Original post by SunTiger)
    I know the phases, stationary, mobile etc... Also the retention time can show which drug is eluted faster, but how can I match the drug to the retention time? Am I missing something? Googleing didn't help much.
    The three drugs do not have the same intermolecular forces. If you used a non-ionic stationary phase then the most polar will elute first, etc.

    Compare the structures of the three pharmaceuticals and think about their intermolecular forces.
 
 
 
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