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Determination of the amount of creatinine in urine

    • Thread Starter

    Hello people,
    I was doing laboratory work on determination of creatinine and had couple of problems. I will list the question and all the details, If you know the answer, please do not hesitate and comment. Thanks in advance!

    It is basically the simple maths question. However, I cannot solve it, or I'm exhausted.

    Question: Calculate the amount of creatinine in daily urine. Is it in normal range?

    Mw(creatinine) = 113.12 g/mol

    Normal amount of creatinine:
    Men 8.8-17.7 mM/24h (if 2000 ml urine)
    Women 5.3-13.3 mM/24h (if 2000 ml urine)

    Results: Light absorbtion = 0.353, so using the graph I got, concentration of creatinine 0.014 mg/0.3ml

    So the question asks if its in the normal range, I know that it is just mixing numbers and getting it in correct units, but I do not know how.. Any help would be appreciated. All I can think of is 2000/0.3 x 0.014 = 93.33 mg/2000ml so 46.65 mg/L. And now is the question how to get mg/L to mM/2000ml and how to use 113.12 g/mol..
    I know its simple question, but any quick and simple answer will help me!

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