i was wondering if anyone can explain how a proton gradient is linked to respiration. this question is from the mark scheme from the exam question below
so basically the first marking point
DNP inhibits respiration by preventing a proton gradient being maintained across membranes. When DNP was added to isolated mitochondria the following changes were observed
• less ATP was produced
• more heat was produced
• the uptake of oxygen remained constant.
Explain how DNP caused these changes.
1. Less / no proton / H+ movement so less / no ATP produced;
2. Heat released from electron transport / redox reactions / energy not used to produce ATP is released as heat;
3. Oxygen used as final electron acceptor / combines with electrons (and protons);
Should I still go?