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Determination of a mystery protease Watch

    • Thread Starter

    For my 3rd year dissertation I was given a mystery protease that I had to find out what it was through a series of experiments.

    I know that it prefers pH 8, and the substrate NSPLN. It's inhibited by PMSF, so from that I've determined that it's a Serine protease. But I don't know how to go from here, is there any way that I can find out the exact protease? Or at least make a reasonable guess?
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Updated: October 14, 2016
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