Can somebody explain me how I can use absorbance to find the concentration of protein in each sample tested. The information I have is the wavelength use, the absorbance for each sample. And the amount of contents in the sample which are protein standard, water, diluted plasma, control sample, biuret reagent.
I just need at explanation to how and why it is done like that and then i'll be able to to my calculations on my own
Thanks in advance!
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