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Absorbance and Concentration Watch

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    Can somebody explain me how I can use absorbance to find the concentration of protein in each sample tested. The information I have is the wavelength use, the absorbance for each sample. And the amount of contents in the sample which are protein standard, water, diluted plasma, control sample, biuret reagent.
    I just need at explanation to how and why it is done like that and then i'll be able to to my calculations on my own

    Thanks in advance!
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    Have you looked up the Beer-Lambert law? That should help.
 
 
 
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