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    how to calculate ec50 on excel?
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    Place your values of response magnitude (or degree of effect of drug e.g. change in heart rate) (which you should standardize so that the maximum effect is 100 %) in one column and the dose of drug in the previous column.

    Then select all the cells in those two columns and do Insert > Chart > Line Chart > Scatter Chart (drug dose on x axis; effect y axis)

    Then draw a horizontal line rightwards from the 50% mark on the y axis and read off the EC50 on the x axis

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