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    • Thread Starter

    1) Is this correct: (my notes)

    Most images produced by LM is usually low in contrast. This is because most components of the object doesn't absorb light (its transparent.)

    For example: The components within cells are mostly transparent (.g. cytosol - liquid interrior) therefore they cant absorb light at diferent degrees, hence the image of the cell produced is low in contrast.

    We use dyes called stains to bind to specific components within cells at different degrees. Therefore the use of staining improves the contrast of images as it makes the object components more visible - so the cells can be identified.

    2) Why do we air dry the slide and heat fix it? (I dont get that part in the book)

    3) Do we need to know the procces of LM in detail, e.g. that the condensor lens focuses the light onto the specimen etc...

    or this:
    Visible light passes throught the specimen, which is filtered and produces an image due to the components absorbing the light at different levels.
    The lenses then magify the images and produces an image through the eyepiece les.

    4) What are photomicroghraphs?

    Sorry for the long questions
    • Thread Starter

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