When you're doing an experiment do you need to do a control reaction or something? we're doing a reaction with enzymes to see how temp affects the rate and our teacher said something about also doing the experiment without adding the enzyme or denaturing the enzyme first. do you know why and how?
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control variabe - enzyme reactions watch
- Thread Starter
- 06-11-2016 21:15
- 06-11-2016 22:46
The control is a test to find out if there is any background factor affecting your experiment, and see how it acts in the absense of whatever you're testing. For example, if you were testing an enzyme that catalyses decomposition of hydrogen peroxide to find the volume of gas produced, you would do the control with no enzyme added to see how much gas would be produced anyway, due to external factors like temperature and stirring. This is especially important in trials with people involved, to see if things like the placebo effect are involved.
You should know from GCSE biology that any enzyme can be denatured by a high temperature above its optimum, boiling should do it. You could also just add distilled water or something instead of enzyme solution, if your enzymes are in a solution.