Why is Chromatography carried out in the dark?

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JJV17
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Why is Chromatography carried out in the dark?, Separation of photosynthetic pigments
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lizardlizard
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Think about what happens to the photosynthetic pigments when exposed to light, and could change their mass. Why would this be a bad thing during chromatography?
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JJV17
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(Original post by lizardlizard)
Think about what happens to the photosynthetic pigments when exposed to light, and could change their mass. Why would this be a bad thing during chromatography?
Thanks, Im not really sure what happens to the photosynthetic pigments when exposed to light, but you stating about their mass, maybe they could gain or loose mass? Therefore, gaining mass would be bad as they wont be able to travel up the chromatography plate higher than they should? therefore affecting their Rf value? vise versa with loosing mass.
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lizardlizard
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During photosynthesis, the photosystems are photoionised (ionised by light) which can affect their mass, I'm not sure what the exact ionisation reaction is but it would change the mass of the photosystems depending on if they were ionised or not. You also might have some functioning structures like electron transport chains remaining in your chromatography paper - although there will obviously be very little energy generation, these could start reating molecules like water into hydrogen ions and oxygen, forming gases and affecting your chromatography.
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JJV17
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(Original post by lizardlizard)
During photosynthesis, the photosystems are photoionised (ionised by light) which can affect their mass, I'm not sure what the exact ionisation reaction is but it would change the mass of the photosystems depending on if they were ionised or not. You also might have some functioning structures like electron transport chains remaining in your chromatography paper - although there will obviously be very little energy generation, these could start reating molecules like water into hydrogen ions and oxygen, forming gases and affecting your chromatography.
Ok many thanks!
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lukeschoield20
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(Original post by lizardlizard)
Think about what happens to the photosynthetic pigments when exposed to light, and could change their mass. Why would this be a bad thing during chromatography?
nono, the answer to why it's run in the dark is that otherwise the pigments would fade in the light and wouldn't be easily identifiable
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