In an MTT assay, if there is a reduction in cell viability in a drug-treated sample of cells compared to untreated cells (control) could you say that the MTT assay results show increased apoptosis in the treated sample? I am testing to see whether 2 drugs (inhibitor of proliferative kinases and an inhibitor of PARPs) reduce cell viability using an MTT assay.
MTT assay interpretation Watch
- Thread Starter
- 05-01-2017 19:20
- 08-01-2017 02:08
MTT is normally reduced by certain energy-rich molecules e. g. NADH - if this reduction fails to occur due to "poisoning" of these compounds (which are essential to life of cells), then yes, they would lead to cell death [weather you label it apoptosis or necrosis); therefore the colorimetric assay of MTT will provide an indication of the degree of cell death.