I need help with these questions for the Quantitative Benedict's procedure.
1. Explain the colour change in Benedict's solution in terms of copper ions and electrons.
2. Explain how the absorbance (or % transmission) readings relate to glucose concentration.
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Quantitative Bendict's Procedure watch
- Thread Starter
- 25-10-2017 17:45
- 25-10-2017 18:22
The Benedict’s solution contains copper (ll) sulphate, when it gains an electron from the reducing sugar it is reduced to copper (l) oxide. If the glucose concentration is higher, there are more glucose molecules so more electrons available to be donated. This means that the higher the concentration, the more copper ions are reduced and the redder the colour