Why do we squash the root tip? Watch

Bertybassett
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in the famous biology practical, why do we squash the root tip. i used to thing it was to make the tissue thin to allow light to pass through it to allow us to see, but after a test today the mark scheme said to make a single celled layer - which is correct and why is it nescessary to have a single celled layer? many thanls
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Bertybassett
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anyone
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have
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So you don't see lots of layers on top of each other,
If it wasn't one cell thick, you would just see cells everywhere and can't actually see the structure.
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Lilith2400
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Bertybassett
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sorry?
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lucyaskew
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I think it's also so that you reduce the numbers of organelles that are together and in front of each other so you can see things clearer.
You are right though.
It needs to be one cell thick as light from the light source/mirror must be able to pass through the sample into the eyepiece. If there is no light, you won't see anything.
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moonwilt
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You are also correct! Having a single layer of cells means light will be able to pass through so the cell structures and organelles will be visible when viewing them through an optical microscope. Having a single layer also means you can view the structures clearly, as there won't be many layers overlapping, which would make it difficult to see a single cell and the organelles.
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Lilith2400
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(Original post by Bertybassett)
sorry?
I was going to say that I think what you said is correct but then I realised you said it wasn't on the markscheme. However, it is in my textbook.
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