Sam1024
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Can anyone please help me on what to include for the evaluation about the root tip.
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SummerStrawberry
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What are you evaluating exactly - is there a specific question you've been asked to answer? Are you evaluating your practical method? Are you evaluating your 'individual performance' against the CPAC?
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chocolateoreo99
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Some random evaluation points I have found (assuming it is the same practical I did, observing mitosis?):

- Human error in counting the number of cells affecting results

- Not left in solution for long enough for successful staining

- Microscope resolution



Hope this is somewhat helpful.
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Sam1024
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(Original post by SummerStrawberry)
What are you evaluating exactly - is there a specific question you've been asked to answer? Are you evaluating your practical method? Are you evaluating your 'individual performance' against the CPAC?
I am evauluating my practical method, as i am unsure on what to include. I have attached my practical method below:

  1. 1. Firstly, you have to stand the beaker on a bench mat before adding (approximately) 10ml of Hydrochloric Acid ( 5 mol dm-3)
  2. 2. Secondly, place about 2 cm3 of the root tip in the acid and leave it in there for 15 minutes.
  3. 3. Next, set up the microscope whilst you are waiting.
  4. 4. After that, you will need to rinse the root tip in distilled water in the watch glass.
  5. 5. Moreover, cut off the root tip (approximately 1mm) and place it on the microscope slide.
  6. 6. In addition, you will need to cover the section with toluidine blue stain and macerate with the mounted needle to separate the cells.
  7. 7. Furthermore, you will continue to macerate until the tissue is well broken and the cells are stained in dark blue.
  8. 8. Next, add a cover slip and with gentle finger pressure ‘spread’ the material and blot at the same time by using a folded filter paper between the finger and the slide.

Finally, look carefully at all slides for the cells undergoing mitosis. As the chromosomes should be stained dark blue. This will need to be repeated for several fields of views.

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Sam1024
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(Original post by chocolateoreo99)
Some random evaluation points I have found (assuming it is the same practical I did, observing mitosis?):

- Human error in counting the number of cells affecting results

- Not left in solution for long enough for successful staining

- Microscope resolution



Hope this is somewhat helpful.
Thanks
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