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The biuret test
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PODHEC1
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My textbook describes the biuret test as semi-quantitative. But I don't understand how it could be this since it is simply a colour change blue to purple so surely it must be qualitative?
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moolio20
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(Original post by HN786)
My textbook describes the biuret test as semi-quantitative. But I don't understand how it could be this since it is simply a colour change blue to purple so surely it must be qualitative?
My textbook describes the biuret test as semi-quantitative. But I don't understand how it could be this since it is simply a colour change blue to purple so surely it must be qualitative?
Hope this helps to explain it.
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username2613789
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Its qualitative because you can look at the colours and be happy about their shades and such.
Its quantitative cause you can measure that you needed 20.5 cm^3 of "Chemical" to observe the happy shades.
Its quantitative cause you can measure that you needed 20.5 cm^3 of "Chemical" to observe the happy shades.
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PODHEC1
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(Original post by moolio20)
You can measure the concentration of the protein present in your sample by observing the intensity of the colour change. One way to do this is measure the absorbance of the sample. Those containing more proteins will give a greater value. This is because of the gradient of the colour change. It can be faint or quite intense.
Hope this helps to explain it.
You can measure the concentration of the protein present in your sample by observing the intensity of the colour change. One way to do this is measure the absorbance of the sample. Those containing more proteins will give a greater value. This is because of the gradient of the colour change. It can be faint or quite intense.
Hope this helps to explain it.
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moolio20
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#5
(Original post by tremen222)
Its qualitative because you can look at the colours and be happy about their shades and such.
Its quantitative cause you can measure that you needed 20.5 cm^3 of "Chemical" to observe the happy shades.
Its qualitative because you can look at the colours and be happy about their shades and such.
Its quantitative cause you can measure that you needed 20.5 cm^3 of "Chemical" to observe the happy shades.
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moolio20
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#6
(Original post by HN786)
What about the iodine test that must be qualitative?
What about the iodine test that must be qualitative?
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