Urgent please help how do i identify banding patterns in electrophoresis

Watch
sarahjuan
Badges: 1
Rep:
?
#1
Report Thread starter 2 years ago
#1
Q4) Referring to lane 2 of the figure shown in Question 5, what is the identity of the nucleic acid indicated by the arrow labelled Z? (Z refers to the smear seen in lane 2 of the figure.) Link to image https://drive.google.com/file/d/1048...ew?usp=sharing Transfer RNA (tRNA) Mitochondrial DNA Ribosomal RNA (rRNA) Nuclear (chromosomal) DNA Messenger RNA (mRNA) Degraded RNAQ5 the figure below shows a nucleic acid preparation from yeast, following agarose gel electrophoresis and staining. The large arrow on the right indicates the direction of movement of nucleic acid during electrophoresis. Link to image https://drive.google.com/file/d/1048...ew?usp=sharing What is the identity of the nucleic acid indicated by the arrow labelled X? Transfer RNA (tRNA) Messenger RNA (mRNA) Degraded RNA Ribosomal RNA (rRNA) Mitochondrial DNAQ6) Q6) Referring to lane 1 of the figure shown in Question 5, what is the identity of the nucleic acid indicated by the arrows labelled Y? SAME IMAGERibosomal RNA (rRNA) Degraded RNA Nuclear (chromosomal) DNA Messenger RNA (mRNA) Transfer RNA (tRNA) Mitochondrial DNAQ7) q7) The figure below shows a nucleic acid preparation from yeast, following agarose gel electrophoresis and staining. The large arrow on the right indicates the direction of movement of nucleic acid during electrophoresis. NEW IMAGE!, THE REST USE THE SAME https://drive.google.com/file/d/1LwX...ew?usp=sharing The arrows labelled X and Z, What is the identity of the nucleic acid indicated - in the figure below - by the arrow labelled Y?Transfer RNA (tRNA)Messenger RNA (mRNA)Ribosomal RNA (rRNA)Mitochondrial DNANuclear (chromosomal) DNADegraded RNAq8) During the gel electrophoresis of nucleic acids, both DNA and RNA move in which direction?Towards the cathode, because the nucleic acids are positively chargedTowards the anode, because the nucleic acids are negatively chargedTowards the anode, because the nucleic acids are positively chargedTowards the cathode, because the nucleic acids are negatively chargedTHANKS SO MUCH!
0
reply
sarahjuan
Badges: 1
Rep:
?
#2
Report Thread starter 2 years ago
#2
0
reply
blawan
Badges: 14
Rep:
?
#3
Report 2 years ago
#3
Unfortunately don't have the energy to do your homework for you but I understand it can be a tricky topic so I've linked some websites to help you.

https://www.thermofisher.com/uk/en/h...-overview.html

http://www.biotec.uniba.it/area_doce...rophoresis.PDF
0
reply
sarahjuan
Badges: 1
Rep:
?
#4
Report Thread starter 2 years ago
#4
Hello
Thanks for replying, its a test due by tomorrow midnight and i have spent over 5 hours on research and trying to look up the banding patterns. I cannot find it anywhere and just feel like guessing at this point. i know what the process is. still cant find out the patterns. We used chromosomal dna from yeast, so would all the bandings just be chromosomal dna?
please help me
0
reply
sarahjuan
Badges: 1
Rep:
?
#5
Report Thread starter 2 years ago
#5
if you could even help with one or two questions it would mean the world to me!
0
reply
X

Quick Reply

Attached files
Write a reply...
Reply
new posts
Back
to top
Latest
My Feed

See more of what you like on
The Student Room

You can personalise what you see on TSR. Tell us a little about yourself to get started.

Personalise

Current uni students - are you thinking of dropping out of university?

Yes, I'm seriously considering dropping out (94)
13.78%
I'm not sure (32)
4.69%
No, I'm going to stick it out for now (214)
31.38%
I have already dropped out (16)
2.35%
I'm not a current university student (326)
47.8%

Watched Threads

View All