What is the puropose of aseptic techniques and sterilisation in the lab?

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Bertybassett
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e.g. when growing bacteria on agar, why do you have to sterilise everything? my book says to prevent contaimination with microbes, but why it it important to not contaminate it with microbes? many thanks
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bean101
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Because then the strain of bacteria you are trying to grow will not be pure and could cause implications with whatever you’re using the bacteria for e.g. using it in recombinant DNA technology to produce insulin from it could cause the insulin to be contaminated and unsafe for human use etc.
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Bertybassett
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(Original post by bean101)
Because then the strain of bacteria you are trying to grow will not be pure and could cause implications with whatever you’re using the bacteria for e.g. using it in recombinant DNA technology to produce insulin from it could cause the insulin to be contaminated and unsafe for human use etc.
i'm not sure what you mean by this? if we were trying to see which disinfectant or antibiotic had the biggest zone of inhibition when applied to bacteria etc, why would we need to carry out aseptic techniques?
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bean101
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(Original post by Bertybassett)
i'm not sure what you mean by this? if we were trying to see which disinfectant or antibiotic had the biggest zone of inhibition when applied to bacteria etc, why would we need to carry out aseptic techniques?
Because you’d want to make sure that it’s only bacteria and not any contaminant because then it wouldn’t show the true effect of the disinfectant
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Bertybassett
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(Original post by bean101)
Because you’d want to make sure that it’s only bacteria and not any contaminant because then it wouldn’t show the true effect of the disinfectant
thanks. so it is not anything to do with safety?
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bean101
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(Original post by Bertybassett)
thanks. so it is not anything to do with safety?
I mean not really as anything grown on agar is safely disposed of anyway so
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