Steps for making protein using DNA technologyWatch this thread
Step 1: Isolation of the DNA fragments that have the gene for the desired protein.
To produce DNA fragments, we use reverse transcriptase or restriction endonucleases or the gene machine.
After that, we use DNA probes to locate the required gene.
Step2: Growth/cloning of the gene.
Two methods: - In vivo - In vitro
For in vivo:
1. Insertion of the DNA fragment into a vector
2. Transfer DNA into suitable host cells
3. Identification of the host cells that have successfully taken up the gene by use of gene markers
For in vitro:
1. Using PCR
2. Separation of the DNA strand
3. Addition of the primers
4. Synthesis of DNA
Is the above summary correct? I’m really confused of how the whole process works because the book didn’t introduce the methods in order. pls help me!!!