Chromatography help please

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Hi freinds
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#1
Report Thread starter 3 years ago
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(b) A tripeptide, L, is partially hydrolysed with concentrated hydrochloric acid to produce two dipeptides and the amino acids alanine (ala), lysine (lys) and serine (ser).
The two dipeptides are separated by chromatography. The diagram below shows the chromatogram.

The table below contains the Rf values of some dipeptides.

Dipeptide ala-lys ala-ser lys-ser lys-ala ser-ala ser-lys
Rf value 0.55 0.85 0.10 0.20 0.15 0.45
Use the chromatogram in the diagram above and the Rf values in the table to identify the two dipeptides present in spots M and N.
Use your answers to deduce the order of the amino acids in the tripeptide L.
Dipeptide responsible for spot M ________________________________________
___________________________________________________________________
Dipeptide responsible for spot N _________________________________________
___________________________________________________________________
Order of amino acids in tripeptide L ______________________________________
___________________________________________________________________
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Hi freinds
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#2
Report Thread starter 3 years ago
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this is the diagram
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