Helpp123
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#1
Report Thread starter 2 years ago
#1
Has anyone created a method for measuring the respiration rate in yeast for PAG 12? I'm stuck

Thanks!
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Lauryyys
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#2
Report 1 year ago
#2
(Original post by Helpp123)
Has anyone created a method for measuring the respiration rate in yeast for PAG 12? I'm stuck

Thanks!
Hey did you get there in the end? I'm stuck now.
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cmckennaa
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#3
Report 1 year ago
#3
1. Set up apparatus for waterbath and a CO2 gas collector using a delivery tube
2. Pour 200cm3 of water into a 500cm3 beaker
3. Measure the temperature of the water until it reaches 50ºC
4. Pour 400cm3 of water into a 500cm3 beaker and place a delivery tube underneath a 50cm3 measuring cylinder filled with water and placed vertically facing downwards
5. Pour 10cm3 of yeast into a test tube and add 5cm3 of glucose solution, covering the top with the bung with the delivery tube attached
6. Place the test tube into the waterbath
7. Count the bubbles produced per minute / Measure the time taken for the water level to drop to 25cm3
8. Repeat steps 5-7 with temperatures 40ºC, 30ºC, and 20ºC
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XxThatFemaleXx
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#4
Report 1 year ago
#4
thanks i was so stuck too really appreciate it!
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