PAG 12 OCR A Biology

Helpp123

7

Has anyone created a method for measuring the respiration rate in yeast for PAG 12? I'm stuck

Thanks!

Reply 1

Lauryyys

10

Original post by Helpp123

Has anyone created a method for measuring the respiration rate in yeast for PAG 12? I'm stuck

Thanks!

Hey did you get there in the end? I'm stuck now.

Reply 2

cmckennaa

11

1. Set up apparatus for waterbath and a CO2 gas collector using a delivery tube
2. Pour 200cm3 of water into a 500cm3 beaker
3. Measure the temperature of the water until it reaches 50ºC
4. Pour 400cm3 of water into a 500cm3 beaker and place a delivery tube underneath a 50cm3 measuring cylinder filled with water and placed vertically facing downwards
5. Pour 10cm3 of yeast into a test tube and add 5cm3 of glucose solution, covering the top with the bung with the delivery tube attached
6. Place the test tube into the waterbath
7. Count the bubbles produced per minute / Measure the time taken for the water level to drop to 25cm3
8. Repeat steps 5-7 with temperatures 40ºC, 30ºC, and 20ºC

Reply 3

XxThatFemaleXx

5

thanks i was so stuck too really appreciate it! :smile:

Reply 4

16tahrah

1

I dont fully understand that method could you rewrite it in a simpler way for me pls.

Reply 5

Blue3542

11

Original post by Helpp123

Has anyone created a method for measuring the respiration rate in yeast for PAG 12? I'm stuck

Thanks!

For ocr a Biology PAG 12 is a research practical could you please tell me what that entails asI have heard to research a topic outside of the spec but another teacher told me to researchone of the three given topics on the spec, and when I have the topic or get given it how wouldI research it would I bring my own resources like a textbook into the lab and write it in a bookor online?

Reply 6

Blue3542

11

Original post by 16tahrah

I dont fully understand that method could you rewrite it in a simpler way for me pls.

For ocr a Biology PAG 12 is a research practical could you please tell me what that entails asI have heard to research a topic outside of the spec but another teacher told me to researchone of the three given topics on the spec, and when I have the topic or get given it how wouldI research it would I bring my own resources like a textbook into the lab and write it in a bookor online?

Reply 7

confuseddork

5

does anyone have any good sources for changing temperatures rather than sucrose concentration? everything i've found seems to only change concentration but I would rather do temperature changes as in the post here

Reply 8

RiviniS48new

1

Original post by Blue3542

For ocr a Biology PAG 12 is a research practical could you please tell me what that entails asI have heard to research a topic outside of the spec but another teacher told me to researchone of the three given topics on the spec, and when I have the topic or get given it how wouldI research it would I bring my own resources like a textbook into the lab and write it in a bookor online?

Hi, there are three PAG options, 12.3 which is investigating how a factor of your choice affects photosynthesis in pondweed, 12.2 which is genetic crosses of fruit flies and recording phenotype observations, and 12.1 which is similar to 12.3 but with respiration in yeast broth cultures. They are all from topics on the A2 spec but yes, you do have to write a method yourself and show evidence of independent research. I think it's recommended that you use at least three different sources. We did 12.1 and 12.3 and we put all our research into the 'intro' about why we chose to investigate the factor and which other factors affect the rate of ... Remember to cite it as per the OCR Practical skills handbook and you should be fine. I typed it but I think it's up to you - check with your teacher in case they prefer you to submit your method and writeup in a particular format, though. I think it's recommended you use a mix of online and offline sources - for offline I used pdfs of studies and trials that had been uploaded online, and cited the paper reference with the journal and everything.
Hope this helps and best of luck with it all! :smile:

(edited 4 months ago)

Reply 9

RiviniS48new

1

Original post by RiviniS48new

Hi, there are three PAG options, 12.3 which is investigating how a factor of your choice affects photosynthesis in pondweed, 12.2 which is genetic crosses of fruit flies and recording phenotype observations, and 12.1 which is similar to 12.3 but with respiration in yeast broth cultures. They are all from topics on the A2 spec but yes, you do have to write a method yourself and show evidence of independent research. I think it's recommended that you use at least three different sources. We did 12.1 and 12.3 and we put all our research into the 'intro' about why we chose to investigate the factor and which other factors affect the rate of ... Remember to cite it as per the OCR Practical skills handbook and you should be fine. I typed it but I think it's up to you - check with your teacher in case they prefer you to submit your method and writeup in a particular format, though. I think it's recommended you use a mix of online and offline sources - for offline I used pdfs of studies and trials that had been uploaded online, and cited the paper reference with the journal and everything.
Hope this helps and best of luck with it all! :smile:

These were my sources for 12.3 (the photosynthesis one):
[1] Sue Hocking, Frank Sochacki, Mark Winterbottom (2015), OCR A Level Biology A, 2nd ed., Glasgow, Bell and Bain Ltd.
[2] Stefan Timm, Martin Hagemann (2020), ‘Photorespiration—how is it regulated and how does it regulate overall plant metabolism?’, Journal of Experimental Botany, Volume 71, Issue 14, Pages 3955–3965
[3] Michael E. Salvucci, Katherine W. Osteryoung, Steven J. Crafts-Brandner, Elizabeth Vierling (2001), ‘Exceptional Sensitivity of Rubisco Activase to Thermal Denaturation in Vitro and in Vivo’, Plant Physiology, Volume 127, Issue 3, November 2001, Pages 1053–1064

And these were my sources for 12.1 (respiration one):
[1] H. Janssens, L. Kim, I. Lee, M. Salehzadeh, 2016, Effect of varying temperature on the rate of CO2 production in baker’s yeast (Saccharomyces cerevisiae), The Expedition, Vol. 5, 2015
[2] J.M. Berg, J.L. Tymoczko, L. Stryer, 2002,Glycolysis is an energy-conversion pathway in many organisms, Biochemistry, W H Freeman, New York (2002)
[3] H.Gupta, Factors Affecting the Rate of Aerobic Respiration: 10 Factors, [Internet], biology.com, c2014, Date last accessed 2023 November 5, Available from https://www.biologydiscussion.com/respiration/aerobic-respiration/factors-affecting-the-rate-of-aerobic-respiration-10-factors/18079

My methods and writeups were passed so I think these are valid sources, in case it's helpful :smile:

PAG 12 OCR A Biology

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