Ailurophile03
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i thought the answer would be 1 and 4 but it is 2 and 3
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Ailurophile03
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bobby147
thank youuuu
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cee.cee
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Because it's the opposite base it fluoresces. So take the sequence (for example GTCCATG), translate to the sequence of opposite bases (CAGGTAC) and compare the answers to the second sequence you've translated
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bobby147
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(Original post by Ailurophile03)
i thought the answer would be 1 and 4 but it is 2 and 3
Most important thing to do since you know the DNA sequence of the sample ,is to write out the complementary sequence to that DNA sequence.Which is
ACCAGTTCTAATA.
So for the probe to bind ,it has to have a part of this complementary sequence .e.g. TAATA,GTTC,ACCA etc.
If we look at the sequences of the DNA probe only two would bind.

DNA probe for spot 3 is the first 6 letters of our complementary sequence and DNA probe for spot 2 is the last 6 letters of our complementary sequence .Only those would bind to our original DNA sample.The other two aren’t complementary.

Hope this helps.
Embedded is just how you find the complementary sequence.
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Ailurophile03
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(Original post by bobby147)
Most important thing to do since you know the DNA sequence of the sample ,is to write out the complementary sequence to that DNA sequence.Which is
ACCAGTTCTAATA.
So for the probe to bind ,it has to have a part of this complementary sequence .e.g. TAATA,GTTC,ACCA etc.
If we look at the sequences of the DNA probe only two would bind.

DNA probe for spot 3 is the first 6 letters of our complementary sequence and DNA probe for spot 2 is the last 6 letters of our complementary sequence .Only those would bind to our original DNA sample.The other two aren’t complementary.

Hope this helps.
Embedded is just how you find the complementary sequence.
thank you very much❤️
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