Lab Calculation QuestionsWatch
You are required to make up 1L of a standard experimental solution comprising of in mM: NaCl 130, KCl 5, MgCl2 1.2, CaCl2 1.5, D-Glucose 8 and HEPES 10; pH 7.4. Explain how you would go about achieving this.
Calculate how much of each constituent chemical you would require.
a. You are required to prepare 1ml of a 50 mM stock of 2-aminoethoxydiphenylborate (Mr = 225) in 100 % DMSO. How much 2-aminoethoxydiphenylborate is required?
b. Using this stock solution, explain how you would prepare 10 mL each of 0.1, 1, 10 and 50 µM of 2-aminoethoxydiphenylborate in the standard experimental solution, all with the same final DMSO concentration of 0.1 % (v/v).
8) Starting with the following stock solutions, which you have to make up:
1 M Tris-HCl, pH 7.5 (Tris base, Mr = 121.14)
0.5 M KCl (KCl, Mr = 74.55)
0.5 M KH2PO4 (KH2PO4, Mr = 136.09)
And 12.3 mg.ml-1 of an antibody solution
a. How you would make 100 mL of 30 mM Tris-HCl (pH8.0), 20 mM KCl and 7 mM KH2PO4 ?
b. how you would make the following series of standards – 10 mL each of 10 µg.mL-1, 100 µg.mL-1 and 1 mg.mL-1 antibody in the above buffer.