Yazomi
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#1
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#1
Hi there, could someone please explain the use of each section of the structure of the tRNA please, the text book only showed the picture and websites doesn’t explains what I am looking for
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macpatgh-Sheldon
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Hi looking at the tRNA molecule as an upright "cloverleaf" shape, the upper end (open end) has the nitrogen base sequence that allows it to attach to a specific amino acid in a lock and key fashion similar to the attachment of an enzyme's active site to the substrate molecule.

The "bottom" end (closed end in a way) is the part that possesses the anti-codon i.e. the triplet sequence of bases complementary to the codon on the mRNA that codes for the same specific amino acid that is able to bind to this particular tRNA.

Another fact to know is that a tRNA molecule has approx. 80 nucleotides (you will know that a nucleotide consists of a nitrogen base, a pentose sugar (here ribose) and as phosphate group.
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Yazomi
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#3
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#3
Ahh thank you so much!! Are there no need to know the use of the base pares on the left and right chunk of the tRNA for the exam
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macpatgh-Sheldon
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#4
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Hi again young man - I am not fully conversant with the detailed syllabus for OCR, but I know that they do not expect you to know irrelevant info or info with no practical importance or even incorrect info [like Edexcel do], so it is unlikely that you would need to know the importance of the two "horizontal arms" of the tRNA molecule [with the unpaired bases].

Having said that, if you are aiming for A* or even for out-of-syllabus info for a synoptic essay Q, it might be worth knowing that the above-mentioned arms (called T-stem and D-stem, respectively), help in the identification of the correct amino acid to bind to a specific tRNA [and for the proofreading step that follows this binding], and for correct alignment of the aa-tRNA [aminoacyl-tRNA) synthetase enzyme (dw if you cannot remember this - same goes for the pic I am about to upload - these are degree level pieces of info).

Good luck!
M (specialist biology tutor)
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macpatgh-Sheldon
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#5
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#5
Here's the pic as promised!
Yazomi

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Yazomi
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#6
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(Original post by macpatgh-Sheldon)
Hi again young man - I am not fully conversant with the detailed syllabus for OCR, but I know that they do not expect you to know irrelevant info or info with no practical importance or even incorrect info [like Edexcel do], so it is unlikely that you would need to know the importance of the two "horizontal arms" of the tRNA molecule [with the unpaired bases].

Having said that, if you are aiming for A* or even for out-of-syllabus info for a synoptic essay Q, it might be worth knowing that the above-mentioned arms (called T-stem and D-stem, respectively), help in the identification of the correct amino acid to bind to a specific tRNA [and for the proofreading step that follows this binding], and for correct alignment of the aa-tRNA [aminoacyl-tRNA) synthetase enzyme (dw if you cannot remember this - same goes for the pic I am about to upload - these are degree level pieces of info).

Good luck!
M (specialist biology tutor)
Ah I see, thank you so much! That was really helpful :) it makes sense in my head now
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