Xcyx336
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How to work out Km with competitive inhibition?
Vmax is 200 and inhibition concentration is 3
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lyer_in_hellfyre
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Check out page 2 on this pdf
http://www-plb.ucdavis.edu/courses/b...zKinetics2.pdf
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Xcyx336
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(Original post by lyer_in_hellfyre)
Check out page 2 on this pdf
http://www-plb.ucdavis.edu/courses/b...zKinetics2.pdf
Kmapp is Vmax?
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lyer_in_hellfyre
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(Original post by Xcyx336)
Kmapp is Vmax?
I thought the question was asking abt Kmapp?
Last edited by lyer_in_hellfyre; 2 weeks ago
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Xcyx336
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can you see the graph
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Xcyx336
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(Original post by lyer_in_hellfyre)
I thought the question was asking abt Kmapp?
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This is the equation for the graph V0/ [S]
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Xcyx336
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This answer should be 100 because it's asking in absence of inhibition which is half of Vmax
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lyer_in_hellfyre
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Also this...
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Xcyx336
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(Original post by lyer_in_hellfyre)
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This is the equation for the graph V0/ [S]
so Kmapp is equal to inhibition concentration add substrate concentration at Km
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lyer_in_hellfyre
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(Original post by Xcyx336)
This answer should be 100 because it's asking in absence of inhibition which is half of Vmax
Is the Vmax without inhibitor 200 tho?
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Xcyx336
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(Original post by lyer_in_hellfyre)
Is the Vmax without inhibitor 200 tho?
yes
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(Original post by Xcyx336)
yes
Well then, half v max is 100 as you said...what did you get Km as?
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Xcyx336
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(Original post by lyer_in_hellfyre)
Well then, half v max is 100 as you said...what did you get Km as?
Km is half of Vmax so is 100
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(Original post by lyer_in_hellfyre)
Well then, half v max is 100 as you said...what did you get Km as?
the only thing is I don't know how to get km in present of inhibition
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(Original post by Xcyx336)
Km is half of Vmax so is 100
Km is the substrate concentration at half v max . Half v max is 100
which means Km is 10 μM
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Xcyx336
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(Original post by lyer_in_hellfyre)
Km is the substrate concentration at half v max . Half v max is 100
so now see the graph is 10 for no inhibition and 30 for inhibition
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(Original post by lyer_in_hellfyre)
Km is the substrate concentration at half v max . Half v max is 100
which means Km is 10 μM
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(Original post by Xcyx336)
so now see the graph is 10 for no inhibition and 30 for inhibition
Edit :
Yep 10 μM is Km and 30μM is Km'
Last edited by lyer_in_hellfyre; 2 weeks ago
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Xcyx336
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(Original post by lyer_in_hellfyre)
Yep 10 μM is Km and 30μM is Ki
According to the graph, Km with inhibition should be 30 right?
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