# Help with enzyme kenetic

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2 weeks ago
#21
(Original post by Xcyx336)
According to the graph, Km with inhibition should be 30 right?
LIH has given you a good explanation there
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2 weeks ago
#22
Ref :bbc
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#23
(Original post by lyer_in_hellfyre)
Ref :bbc
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#24
(Original post by lyer_in_hellfyre)
Ref :bbc
Think now are correct
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2 weeks ago
#25
(Original post by Xcyx336)
Think now are correct
(Original post by Xcyx336)
According to the graph, Km with inhibition should be 30 right?

Hey i edited my earlier post so.. 30μM is Km apparent
For Km, 10μM to 1 sf is 10μM
And for the last....
Last edited by lyer_in_hellfyre; 2 weeks ago
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#26
(Original post by lyer_in_hellfyre)
I thought the question was asking abt Kmapp?
Question itself is asking about two Km one is absence of inhibition and one is presence of inhibition and finally it's asking for Ki
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#27
(Original post by lyer_in_hellfyre)
Hey i edited my earlier post so.. 30μM is Km apparent
For Km, 10μM to 1 sf is 10μM
And for the last....
Hold on. We are now sure that Km without inhibition is definitely 10 and with inhibition is 30 according to the graph
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2 weeks ago
#28
(Original post by Xcyx336)
Question itself is asking about two Km one is absence of inhibition and one is presence of inhibition and finally it's asking for Ki
Yep so now we have got values for Km, Kmapp, concentration of inhibitor...now just plug in to this equation
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#29
(Original post by lyer_in_hellfyre)
Hey i edited my earlier post so.. 30μM is Km apparent
For Km, 10μM to 1 sf is 10μM
And for the last....
But for Ki we have to know Kmapp first. The Kmapp should be 33?
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#30
(Original post by lyer_in_hellfyre)
Yep so now we have got values for Km, Kmapp, concentration of inhibitor...now just plug in to this equation
How do you know the Kmapp is 30?
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2 weeks ago
#31
(Original post by Xcyx336)
Hold on. We are now sure that Km without inhibition is definitely 10 and with inhibition is 30 according to the graph
Yeah.. in μM
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#32
(Original post by lyer_in_hellfyre)
Yeah.. in μM
now we need to use this equation to find out Ki
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2 weeks ago
#33
(Original post by Xcyx336)
How do you know the Kmapp is 30?
Kmapp is the substrate concentration at half vmax in the graph with inhibitor
Km apparent is 30μM
Last edited by lyer_in_hellfyre; 2 weeks ago
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#34
(Original post by lyer_in_hellfyre)
Yeah.. in μM
The question is i don't know Ki and Kmapp at this stage
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#35
(Original post by lyer_in_hellfyre)
Kmapp is the substrate concentration at half vmax in the graph with inhibitor
So it's 30?
(Original post by lyer_in_hellfyre)
Kmapp is the substrate concentration at half vmax in the graph with inhibitor
now it's like this?
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2 weeks ago
#36
(Original post by Xcyx336)
now we need to use this equation to find out Ki
Yes, make Ki the subject
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#37
(Original post by lyer_in_hellfyre)
Yes, make Ki the subject
What is the value of Km in this equation?
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2 weeks ago
#38
(Original post by Xcyx336)
now it's like this?
You've got it good almost but...
Is Km 30?
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2 weeks ago
#39
(Original post by Xcyx336)
What is the value of Km in this equation?
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#40
(Original post by lyer_in_hellfyre)
You've got it good almost but...
Is Km 30?
Km is 100 at this point?
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