In my IB Extended Essay experiment (in chemistry) I looked at factors influencing liver catalase, one of which was salt concentration.
I have read that at high concentrations, normal interactions within the catalase molecule are blocked, and new interactions form (presumably with the NaCl?). I need to know, however, what exactly is going on, in greater detail.
Could someone please give me some idea as to just what kind of interactions are being blocked that causes the enzyme's structure to change?
This is one of the main parts of my essay, and I'm having trouble understanding what is going on at the moment!
Thanks a lot,
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Extended essay help needed: Catalase interaction with NaCl watch
- Thread Starter
- 22-08-2008 22:10
- 22-08-2008 22:27
Enzymes are proteins and proteins fold into a three dimensional shapes (specifically secondary and tertiary structures) via inter- and intramolecular forces; for example hydrogen bonding. Increasing salt concentration disrupts some of these interactions, causing the protein to unfold and therefore lose its activity.